Generator

Part:BBa_K1179022:Design

Designed by: Lauren St. Hilaire   Group: iGEM13_MIT   (2013-09-15)


CAG_Acyl-TyA-rtTA3


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1730
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1730
    Illegal BglII site found at 2473
    Illegal XhoI site found at 12
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1730
    Illegal XbaI site found at 1634
    Illegal PstI site found at 2373
    Illegal PstI site found at 2622
    Illegal PstI site found at 2644
    Illegal NgoMIV site found at 1322
    Illegal NgoMIV site found at 1479
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Not applicable

Source

The TyA protein is a yeast viral protein and when added with the Acylation tag it acts as an exosome export protein, allowing the eGFP to be exported when fused to the TyA. Constructed the part by doing a gateway reaction with a CAG promoter and Acyl-TyA-rtTA

References